abstract：:A Na/Ca exchange current can be elicited in voltage clamped single ventricular myocytes by the abrupt removal of extracellular Na+ by means of a rapid switcher device. We measured this reverse Na/Ca exchange current in isolated mouse ventricular myocytes from wild-type mice, and from transgenic mice with hearts overex...

journal_title：Journal of molecular and cellular cardiology

authors： Su Z,Bridge JH,Philipson KD,Spitzer KW,Barry WH

更新日期：1999-05-01 00:00:00

abstract：:An efficient cardiac gene transfer technique in murine models would greatly facilitate the elucidation of the pathophysiology of cardiomyopathies and the development of genetic therapies. Direct myocardial injection or catheter-based intracoronary infusion is not easily achievable in mice and resultant transgene expre...

journal_title：Journal of molecular and cellular cardiology

authors： Zhang JC,Woo YJ,Chen JA,Swain JL,Sweeney HL

更新日期：1999-04-01 00:00:00

abstract：:The contribution of endogenous NO to ischemia-reperfusion injury was studied in isolated perfused hearts of wild-type (WT) and endothelial NO synthase knockout (eNOS-) mice. The hearts were subjected to a 16-min period of global no-flow ischemia and were subsequently reperfused for 1 h. Cardiac contractile function wa...

journal_title：Journal of molecular and cellular cardiology

authors： Flögel U,Decking UK,Gödecke A,Schrader J

更新日期：1999-04-01 00:00:00

abstract：:It is still a matter of debate, whether decreased protein expression of SERCA 2a and phospholamban (PLB), or alterations in the phosphorylation state of PLB are responsible for the reduced SERCA 2a function in failing human myocardium. Thus, in membrane preparations from patients with terminal heart failure due to idi...

journal_title：Journal of molecular and cellular cardiology

authors： Schwinger RH,Münch G,Bölck B,Karczewski P,Krause EG,Erdmann E

更新日期：1999-03-01 00:00:00

abstract：:The kappa-opioid receptor exerts a negative modulatory action on the beta-adrenoceptor and the action is blunted in adult spontaneously hypertensive rats (SHR). In order to determine whether the blunted negative modulation of the beta-adrenoceptor by the kappa-opioid receptor contributes to the development of hyperten...

journal_title：Journal of molecular and cellular cardiology

authors： Yu XC,Wang HX,Zhang WM,Wong TM

更新日期：1999-03-01 00:00:00

abstract：:The polyunsaturated fatty acids (PUFAs) of the omega 3 series are known to modulate adrenergic functions in ventricular myocytes. This study evaluated the influence of hypoxia duration and PUFA composition on the ability of cultured rat cardiomyocytes in producing alpha- and beta-adrenergic messengers (IPs and cAMP). ...

journal_title：Journal of molecular and cellular cardiology

authors： Delerive P,Oudot F,Ponsard B,Talpin S,Sergiel JP,Cordelet C,Athias P,Grynberg A

更新日期：1999-02-01 00:00:00

abstract：:There is increasing evidence that reactive oxygen species (ROS) contribute to post-ischemic reperfusion injury, but determination of the specific ROS involved has proven elusive. In the present study electron paramagnetic resonance (EPR) spectroscopy was used in vitro to measure the relative quenching of singlet oxyge...

journal_title：Journal of molecular and cellular cardiology

authors： Lee JW,Miyawaki H,Bobst EV,Hester JD,Ashraf M,Bobst AM

更新日期：1999-01-01 00:00:00

abstract：:Oxygen radical-mediated injury has been implicated in the process of cardiac aging. We investigated the tolerance to oxidative stress of hearts from rats of different age. Langendorff-perfused hearts from 3-12-month-old rats received a 30 min infusion of the reactive oxygen specie hydrogen peroxide (H2O2, 60 microM), ...

journal_title：Journal of molecular and cellular cardiology

authors： Abete P,Napoli C,Santoro G,Ferrara N,Tritto I,Chiariello M,Rengo F,Ambrosio G

更新日期：1999-01-01 00:00:00

abstract：:In single guinea pig ventricular cells, genistein, a potent inhibitor of protein tyrosine kinase (PTK), was found to suppress the delayed-rectifier K (IK) current. The present study was carried out to examine the underlying mechanism. Ventricular myocytes were voltage-clamped in the conventional whole-cell mode (36 de...

journal_title：Journal of molecular and cellular cardiology

authors： Washizuka T,Horie M,Obayashi K,Sasayama S

更新日期：1998-12-01 00:00:00

abstract：:Fibroblast growth factor-2 (FGF-2), administered to the isolated rat heart by perfusion and under constant pressure, is protective against ischemia-reperfusion (I-R). Here we have investigated whether FGF-2 cardioprotection: (a) is dependent on flow modulation; (b) is linked to effects on contractility; (c) is mediate...

journal_title：Journal of molecular and cellular cardiology

authors： Padua RR,Merle PL,Doble BW,Yu CH,Zahradka P,Pierce GN,Panagia V,Kardami E

更新日期：1998-12-01 00:00:00

abstract：:The rapid application of caffeine to cardiac myocytes is commonly used to assess changes in the Ca2+ content of the sarcoplasmic reticulum (SR) and to study other parameters of intracellular Ca2+ regulation. Here we examined the effects of rapid caffeine application on membrane potential, intracellular Ca2+, and cell ...

journal_title：Journal of molecular and cellular cardiology

authors： Zaniboni M,Yao A,Barry WH,Musso E,Spitzer KW

更新日期：1998-11-01 00:00:00

abstract：:Cardiac hypertrophy is associated with modifications in Ca2+ transport processes, enzymes of energy metabolism and antioxidant capacity. It is unknown whether these changes occur in infarct-induced hypertrophy with regard to an altered susceptibility to ischemia/reperfusion injury. We examined changes in sarcoplasmic ...

journal_title：Journal of molecular and cellular cardiology

authors： Wagner KD,Geil D,Schimke I,Stauss HM,Lammerich A,Theres H,Pfitzer G,Vetter R,Günther J

更新日期：1998-11-01 00:00:00

abstract：:Since angiotensin-converting enzyme (ACE) produces angiotensin II in the heart, ACE inhibitors may prevent coronary vasoconstriction and increase coronary blood flow. On the other hand, since ACE inhibitors also inhibit kininase II which results in reduced degradation of bradykinin, ACE inhibitors may increase cardiac...

journal_title：Journal of molecular and cellular cardiology

authors： Kitakaze M,Node K,Minamino T,Asanuma H,Ueda Y,Kosaka H,Kuzuya T,Hori M

更新日期：1998-11-01 00:00:00

abstract：:The effect of alpha1-adrenergic stimulation on L-type Ca2+ current (ICa,L) in adult rat ventricular myocytes was investigated using three different methods of current recording. During conventional whole-cell recordings with 5 mm-BAPTA included in the pipette solution, phenylephrine (20 microM) did not increase ICa,L ...

journal_title：Journal of molecular and cellular cardiology

authors： Zhang S,Hiraoka M,Hirano Y

更新日期：1998-10-01 00:00:00

abstract：:Angiotensin II has been shown to be mitogenic in various cell types. In cultured neonatal cardiomyocytes, we have demonstrated that angiotensin II causes hypertrophy, not hyperplasia. However, fetal or neonatal cardiomyocytes exhibit limited proliferation in primary culture, and are mitotically less potent. In order t...

journal_title：Journal of molecular and cellular cardiology

authors： Fukuda K,Izumo S

更新日期：1998-10-01 00:00:00

abstract：:Arterial hypotension can cause cerebral ischemia when the autoregulation of the cerebral circulation is exhausted. We hypothesized that sudden cerebral vasoconstriction induced by moderate hypotensive, but hemodynamically stable, sustained ventricular tachycardias (MHT-VT) further compromises cerebral blood flow (CBF)...

journal_title：Journal of molecular and cellular cardiology

authors： Hagendorff A,Dettmers C,Danos P,Hümmelgen M,Vahlhaus C,Martin C,Heusch G,Lüderitz B

更新日期：1998-10-01 00:00:00

abstract：:Biglycan, a small dermatan sulphate proteoglycan, has been postulated to interact with other components of the extracellular matrix (ECM), specifically collagens. We hypothesized that biglycan messenger ribonucleic acid (mRNA) is increased in the myocardial infarct zone. Biglycan mRNA expression after acute myocardial...

journal_title：Journal of molecular and cellular cardiology

authors： Yamamoto K,Kusachi S,Ninomiya Y,Murakami M,Doi M,Takeda K,Shinji T,Higashi T,Koide N,Tsuji T

更新日期：1998-09-01 00:00:00

abstract：:Disturbances of cellular calcium homeostasis due to oxidative stress are involved in reperfusion associated phenomena like myocardial stunning and reperfusion induced arrhythmias. This study investigates the effect of the major neutrophil-derived oxidant hypochlorous acid (HOCl) on the l-type calcium current (ICa,L) o...

journal_title：Journal of molecular and cellular cardiology

authors： Hammerschmidt S,Wahn H

更新日期：1998-09-01 00:00:00

abstract：:Diabetes mellitus is associated with an increased incidence and greater severity of primary atherosclerosis as well as restenosis after angioplasty for reasons not yet clear. We have shown previously that insulin and proinsulin-typically elevated in blood in patients with type II diabetes-increase plasma activity of p...

journal_title：Journal of molecular and cellular cardiology

authors： Nordt TK,Sawa H,Fujii S,Bode C,Sobel BE

更新日期：1998-08-01 00:00:00

abstract：:The effects of cytochalasin D, a specific F-actin depolymerizing agent, on Ca2+ transients in rat ventricular cardiomyocytes were investigated. Cytochalasin D (20 microM) significantly slowed decay of Ca2+ transients (tau decay control cells=28.1+/-1.3, n=28tau decay=47.3+/-2.8 ms, n=20, P<0.001). The rising phase of ...

journal_title：Journal of molecular and cellular cardiology

authors： Undrovinas AI,Maltsev VA

更新日期：1998-08-01 00:00:00

abstract：:We examined changes in expression and function of the cardiac Na+, K(+)-pump in a post-infarction rat model of hypertrophy and congestive heart failure (CHF). Myocardial infarction was induced by ligation of the left coronary artery in Wistar rats and hearts were obtained from animals with CHF and from sham operated r...

journal_title：Journal of molecular and cellular cardiology

authors： Semb SO,Lunde PK,Holt E,Tønnessen T,Christensen G,Sejersted OM

更新日期：1998-07-01 00:00:00

abstract：:We studied the presence of collagen degrading enzymes (matrix metalloproteinases, MMPs) in porcine myocardium following ischemia and late reperfusion. In nine pigs, left anterior descending coronary artery was occluded for 6 h followed by reperfusion for 3 h. Six pigs without coronary occlusion served as controls. Aft...

journal_title：Journal of molecular and cellular cardiology

authors： Danielsen CC,Wiggers H,Andersen HR

更新日期：1998-07-01 00:00:00

abstract：:Heat shock (HS) results in the expression of heat shock proteins (hsp) and confers tolerance against subsequent ischemic injury. We examined the extent of myocardial protection in vivo, and determined the level of hsp expression induced by HS as a function of time. Anesthetized rats were subjected to HS by raising cor...

journal_title：Journal of molecular and cellular cardiology

authors： Qian YZ,Shipley JB,Levasseur JE,Kukreja RC

更新日期：1998-06-01 00:00:00

abstract：:The possible ischemia-selective Class III anti-arrhythmic action (selective action potential widening in ischemia) of the IKATP blocker glibenclamide was assessed in anesthetized rabbits during ischemia induced by complete occlusion of a coronary artery. Coronary artery occlusion caused an initial prolongation in mono...

journal_title：Journal of molecular and cellular cardiology

authors： Barrett TD,Walker MJ

更新日期：1998-05-01 00:00:00

abstract：BACKGROUND:Heat shock proteins (hsp) constitute an endogenous stress response that protects cells from injury. Most work on these important proteins has focused on the immediate response to acute stress in cell culture systems and mammalian models of heart disease. Little is known about the expression of the hsps in hu...

journal_title：Journal of molecular and cellular cardiology

authors： Knowlton AA,Kapadia S,Torre-Amione G,Durand JB,Bies R,Young J,Mann DL

更新日期：1998-04-01 00:00:00

abstract：:Glypicans are a group of membrane-bound heparan sulfate proteoglycans (HSPG) that are tissue specific and developmentally regulated. Transcripts for avian glypican are found in endocardial cushions, limb buds, somites and forebrain of early chick embryos. Since avian glypican is not well characterized, the cellular lo...

journal_title：Journal of molecular and cellular cardiology

authors： Niu S,Bahl JJ,Adamson C,Morkin E

更新日期：1998-03-01 00:00:00

abstract：:We previously established a new measuring method of the myocardial O2 consumption of mechanically unloaded rat left-ventricular slices. O 2 consumption of unstimulated myocardium corresponds to basal metabolism. We have found O2 consumption of stimulated myocardium to include basal metabolism and O 2 consumption for C...

journal_title：Journal of molecular and cellular cardiology

authors： Takaki M,Kohzuki H,Kawatani Y,Yoshida A,Ishidate H,Suga H

更新日期：1998-03-01 00:00:00

abstract：:31P-NMR was used to monitor myocardial bioenergetics in compensated and failing SHHF/MCC-fa(cp) (SHF) rat hearts. The SHHF/Mcc-fa(cp) (spontaneous hypertension and heart failure) rat is a relatively new genetic model in which all individuals spontaneously develop congestive heart failure, most during the second year o...

journal_title：Journal of molecular and cellular cardiology

authors： Michael O'Donnell J,Narayan P,Bailey MQ,Abduljalil AM,Altschuld RA,McCune SA,Robitaille PM

更新日期：1998-02-01 00:00:00

abstract：:Limited time resolution has hampered proper evaluation of changes in intracellular Na+ (Na+i) in whole hearts upon post-ischemic reperfusion. In isolated rat hearts perfused at 37 degrees C, we studied the contribution of the Na+-K+ ATPase and the Na+-H+ exchanger to control of Na+i during reperfusion using 23Na NMR a...

journal_title：Journal of molecular and cellular cardiology

authors： Van Emous JG,Schreur JH,Ruigrok TJ,Van Echteld CJ

更新日期：1998-02-01 00:00:00

abstract：:We have recently shown that norepinephrine (NE) pretreatment attenuates Ca2+ overloading in cardiac rat trabeculae during metabolic inhibition (MI) with NaCN (2 mmol/l), and improves contractile recovery during a subsequent recovery period (RP). In the present study, we investigated the effects of the continuous prese...

journal_title：Journal of molecular and cellular cardiology

authors： Musters RJ,van der Meulen ET,van der Laarse WJ,van Hardeveld C

更新日期：1998-02-01 00:00:00

abstract：:23Na and 31P NMR spectroscopy were used to follow intracellular [Na+] ([Na+]i) and energy metabolism in isolated, perfused rat hearts. During 30 min of Ca(2+)-free perfusion no significant change in [Na+]i could be detected, but during a subsequent 45 min period of ischemia [Na+]i rose significantly as expected, from ...

journal_title：Journal of molecular and cellular cardiology

authors： Van Echteld CJ,Van Emous JG,Jansen MA,Schreur JH,Ruigrok TJ

更新日期：1998-01-01 00:00:00

abstract：:The pyrimidine base, orotic acid (OA), improves the function of recently infarcted hearts subjected to global ischemia but its mechanism of action is unclear. Our aims were to examine (i) in normal rats, the effect of OA on pyrimidine levels in plasma, liver and heart; (ii) in rats with normal or infarcted hearts, the...

journal_title：Journal of molecular and cellular cardiology

authors： Richards SM,Conyers RA,Fisher JL,Rosenfeldt FL

更新日期：1997-12-01 00:00:00

abstract：:We tested the effects of the protein kinase C (PKC) inhibitors bisindolylmaleimide (1 microM) and chelerythrine (2 microM) on myocardial ischemia-reperfusion injury in in situ and isolated perfused rabbit hearts. In non-ischemic isolated hearts, bisindolylmaleimide (1 microM) and chelerythrine (2 microM) blocked sn-1,...

journal_title：Journal of molecular and cellular cardiology

authors： Lasley RD,Noble MA,Mentzer RM Jr

更新日期：1997-12-01 00:00:00

abstract：:Redox stress during post-ischemic reperfusion may be the prime signal for processes leading to myocardial remodelling and hypertrophy. Nitric oxide (NO) is antioxidative, antiadhesive for neutrophils (PMN) and antiproliferative. Thus, enhancing endothelial production of NO, e.g. by inhibiting breakdown of endogenous b...

journal_title：Journal of molecular and cellular cardiology

authors： Zahler S,Kupatt C,Möbert J,Becker BF,Gerlach E

更新日期：1997-11-01 00:00:00

abstract：:We investigated whether parathyroid hormone-related peptide (PTH-rP), recently found expressed in the heart, exerts growth and contractile effects on adult cardiomyocytes from rat hearts. Synthetic PTH-rP peptides were used covering either a protein kinase C (PKC)-activating domain [PTH-rP(107-111)], or an adenylate c...

journal_title：Journal of molecular and cellular cardiology

authors： Schlüter KD,Weber M,Piper HM

更新日期：1997-11-01 00:00:00

abstract：:Apoptosis is a potentially important myocardial response to pathology including ischemia and reperfusion. Na-H exchange (NHE) represents an important mechanism for mediating such injury. The present study was done to determine if NHE inhibition can affect early apoptosis in an acute model of ischemia and reperfusion. ...

journal_title：Journal of molecular and cellular cardiology

authors： Chakrabarti S,Hoque AN,Karmazyn M

更新日期：1997-11-01 00:00:00

abstract：:We have tested the hypothesis that thyroid state may influence both the flow of cellular Ca2+ and the myofilament response to Ca2+ by effects on intracellular pH (pHi) and Na+ (Nai+). Single cardiac myocytes isolated from hypothyroid, euthyroid and hyperthyroid animals were loaded with fura-2/AM (Cai2+ probe), BCECF/A...

journal_title：Journal of molecular and cellular cardiology

authors： Wolska BM,Averyhart-Fullard V,Omachi A,Stojanović MO,Kallen RG,Solaro RJ

更新日期：1997-10-01 00:00:00

abstract：:The effects of Cobra venom cardiotoxin (CTX) on the cellular morphology, twitch amplitude and intracellular calcium ([Ca2+]i) of the ventricular myocytes were studied. [Ca2+]i and twitch amplitude were determined with a fluorometric ratio method using Fura-2/AM and Calcium Green-1 as calcium indicators, and a videomic...

journal_title：Journal of molecular and cellular cardiology

authors： Wang HX,Lau SY,Huang SJ,Kwan CY,Wong TM

更新日期：1997-10-01 00:00:00

abstract：:Although several studies have demonstrated that nitric oxide appears to be cardioprotective and endothelin-1 (ET-1) deleterious in myocardial ischemia/reperfusion injury, their interactions in the intact heart are unknown. Therefore, coronary effluent and interstitial fluid ("transudate") levels of ET-1 and cyclic GMP...

journal_title：Journal of molecular and cellular cardiology

authors： Brunner F

更新日期：1997-09-01 00:00:00

abstract：:We evaluated the time course of activation of endocardial endothelial cells and cardiomyocytes in adult rats with pressure overload induced by the abdominal aortic constriction. The silver staining technique for nucleolar organizer region was used to mark an increased transcriptional activity of the cells. An increase...

journal_title：Journal of molecular and cellular cardiology

authors： Horký M,Horský P,Kolár F

更新日期：1997-09-01 00:00:00